Bacterial diversity dynamics of traditional Turkish Ezine Cheese as evaluated by PCR-DGGE and SSCP analysis


SOFU A. , Ekinci F. Y.

INTERNATIONAL JOURNAL OF DAIRY TECHNOLOGY, vol.69, no.4, pp.592-600, 2016 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 69 Issue: 4
  • Publication Date: 2016
  • Doi Number: 10.1111/1471-0307.12311
  • Title of Journal : INTERNATIONAL JOURNAL OF DAIRY TECHNOLOGY
  • Page Numbers: pp.592-600

Abstract

The aim of this study was to evaluate the bacterial ecosystem of milk and Ezine cheese by PCR amplification of the V3 region of the bacterial 16S rRNA gene followed by denaturing gradient gel electrophoresis (DGGE) and by monitoring the bacterial diversity dynamics using PCR single-strand conformation polymorphism (SSCP) analysis. PCR-DGGE analysis revealed that 17 different bands and strains belonging to the Lactococcus lactis group and Streptococcus thermophilus were predominant during manufacturing and ripening. SSCP analysis revealed that the bacterial profiles of the two cheese samples were similar.