In Isparta, lavender is cultivated on an area of 250 hectares. The lavender plants are often used as lavender oil and lavender buds by drying a small amount too. The research was carried out at the Field Crops Laboratory of Agriculture Faculty of Suleyman Demirel University in 2011. The study was conducted with the aim to determine essential oil and its composition in fresh stem flower and dry stemless flower of lavender and lavandin cultivars. Essential oil was obtained by steam distillation method. Composition of essential oil was identified with GC/MS. Statistically significant differences (P <= 0.01) were observed between both the fresh stem flower and dry stemless flower essential oils of lavender and lavandin cultivars. The highest essential oil rate in both fresh stem flower and dry stemless flower was obtained from L. angustifolia var. Silver (1.72 and 7.50%, respectively), the lowest essential oil rate from L. angustifolia var. Munstead (0.13 and 0.67%, respectively) lavender cultivars. Linalool, linalyl acetate, sabinene, cimene, terpineol-4-ol and camphor in essential oils of lavender and lavandin cultivars were determined as the main components. Essential oil components rate in fresh stem flower and dry stemless flower of lavender cultivars ranged from linalool 25.3 and 43.0%, 25.5 and 42.1%, linalyl acetate 3.6 and 37.4%, 8.0 and 47.2%, camphor 0 and 9.8% , 0 and 11.4%, sabinene 1.6 and 19.4%, 3.0 and 15.8%, cimene 2.7 and 11.0%, 1.9 and 8.5% and terpeneol-4-ol 0.6 and 12.9%, 0 and 13.8%, respectively.