In this study, the total phenol, flavonol amounts, antioxidant capacity and antiradical activity of Origanum onites were determined by using different solvents. Total flavonol contents of O. onites were found between 790.52 mgRE/g (U5) to 1250.63 mg RE/g (U3). Antiradical activity values ranged from 624.83 IC50 = mg/ml (U3) to 1080.16 IC50 = mg/ml (S3). Antioxidant capacity values of O. onites were found between 105.18 mgAAE/g (U5) to 161.71 mgAAE/g (U3). Total phenolic substance amount determined 54.57-141.80 mg GAE/g, and ultrasonic water bath device application acetone: water: acetic acid solvent mixture were determined as the most successful extraction. U3 application determined as the most successful application for total flavonoid amount routine equivalent.