Protective efficacy of ramelteon on methotrexate-induced DNA damage
Drug and Chemical Toxicology, vol.48, no.3, pp.514-520, 2025 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 48 Issue: 3
- Publication Date: 2025
- Doi Number: 10.1080/01480545.2024.2375300
- Journal Name: Drug and Chemical Toxicology
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, Chemical Abstracts Core, EMBASE, Environment Index, Food Science & Technology Abstracts, International Pharmaceutical Abstracts, Veterinary Science Database
- Page Numbers: pp.514-520
- Keywords: Comet assay, Genotoxicity, Melatonin analogue, Melatonin receptor agonist, Ramelteon
- Süleyman Demirel University Affiliated: Yes
Abstract
Ramelteon (RMLT) is a melatonin receptor agonist that it has antioxidative and anti-inflammatory effects associated with DNA damage through different mechanisms of action. In this regard, we investigated the potential usefulness of RMLT as a protective agent against methotrexate (MTX)-induced DNA damage. Four groups were constituted from 32 Wistar albino rats: Negative control, RMLT, MTX, and MTX + RMLT. Twenty mg/kg MTX (i.p., single dose) and RMLT 10 mg/kg (oral, 7 days) was administered. Comet assay was used and the parameter %TailDNA was used to detect DNA damage. %TailDNA was 4.90 ± 0.19 in the control group, 7.85 ± 0.33 in the MTX group, 5.49 ± 0.24 in the RMLT group, and 5.86 ± 0.23 in the MTX + RMLT group. While there was a significant increase in DNA damage in the MTX-treated group compared to the control group, there was a significant reduction in DNA damage in the MTX + RMLT group, compared to the MTX group (p < 0.001). In conclusion, it was observed that combined treatment with RMLT significantly reduced MTX-induced DNA damage.