Food of animal origin derived from livestock farming establishments is necessary to meet the needs of human beings. The wastes generated during the production stages of cattle breeding (manure, ground covers, etc.) create a danger to the environment and water resources when they are released to the environment randomly. Therefore, the waste generated by livestock farming establishments should be stored in a controlled manner and should not cause environmental pollution. The research was carried out in the Euphrates basin and covered four provinces (Adiyaman, Sanliurfa, Gaziantep, and Kilis) and 35 districts. In the context of this study, the number of the cattle bred in the establishments in 328 villages which are at least 150 m and at the most 5 km distance to the flow path of the Euphrates was taken into consideration. In the study, Erdas Imagine 9.3 and ArcMAP 10.0 software were used; the number of the cattle and the distance to the rivers were evaluated as a layer. The subject of the study was the investigation of the potential pollution effects of the cattle breeding on the Euphrates River and its tributaries, and it was carried out to determine risky, non-risky and partially risky areas and attract attention to this issue. As a result, it was concluded that 4 districts and villages of Sanliurfa Region would be classified as risky areas, 3 districts and villages of Gaziantep Region would be classified as partially-risky areas, and 3 districts and villages of Adiyaman Region and all districts and villages of Kilis Region would be classified as non-risky areas.