Omega-3 Fatty Acids Attenuate LPS-Induced Acute Kidney Injury via Activation of AMPK/SIRT1/PGC-1α/NRF2/FOXO3 Signaling and Suppression of NF-κB-Mediated Inflammation.
Nutrients, cilt.18, sa.4, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 18 Sayı: 4
- Basım Tarihi: 2026
- Doi Numarası: 10.3390/nu18040618
- Dergi Adı: Nutrients
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, CINAHL, EMBASE, MEDLINE
- Anahtar Kelimeler: apoptosis, Heme oxygenase 1, HSP70, inflammation, Interleukin 6, omega-3 fatty acids, oxidative stress
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- Süleyman Demirel Üniversitesi Adresli: Evet
Özet
Background: Sepsis-induced acute kidney injury (AKI) involves oxidative stress and inflammation. Omega-3 (OMG-3) fatty acids possess antioxidant properties, but their impact on the mitochondrial AMPK/SIRT1/PGC-1α/NRF2/FOXO3 axis in AKI remains unclear. Methods: Thirty-two male Wistar rats were divided into Control, LPS (5 mg/kg), LPS + OMG-3 (500 mg/kg/day), and OMG-3 groups. Renal tissues were analyzed for histopathology, immunohistochemistry (TNF-α, Caspase-3, HSP70), and gene expression (AMPK, SIRT1, PPGC-1α NRF2, FOXO3). Results: LPS caused severe tubular injury, increased TNF-α, Caspase-3, and HSP70 expression, while significantly downregulating the AMPK/SIRT1/PGPGC-1αRF2/FOXO3 signaling pathway. OMG-3 treatment alleviated histopathological damage, suppressed inflammatory and apoptotic markers, and restored the expression of mitochondrial and antioxidant genes. Conclusions: OMG-3 fatty acids protect against LPS-induced AKI by upregulating the gene expression of components in the AMPK/SIRT1/PGCPGC-1αF2/FOXO3 axis and suppressing NF-κB-driven inflammation. This dual regulation highlights OMG-3 as a potential therapeutic agent for sepsis-related renal injury.