Synthesis, Characterization, Antiproliferative Activity, Toxicity, and ADME Studies of a New Schiff Base
Gazi University Journal of Science, vol.39, no.2, pp.905-915, 2026 (ESCI, Scopus, TRDizin)
- Publication Type: Article / Article
- Volume: 39 Issue: 2
- Publication Date: 2026
- Doi Number: 10.35378/gujs.1762528
- Journal Name: Gazi University Journal of Science
- Journal Indexes: Emerging Sources Citation Index (ESCI), Scopus, TR DİZİN (ULAKBİM), Academic Search Ultimate (EBSCO), Biomedical Reference Collection: Corporate Edition (EBSCO), Engineering Source (EBSCO)
- Page Numbers: pp.905-915
- Keywords: Breast cancer, Cytotoxic activity, Protox-II, Schiff base
- Open Archive Collection: AVESIS Open Access Collection
- Süleyman Demirel University Affiliated: Yes
Abstract
In this study, a new Schiff base 3 was synthesized in a reaction step using N1-phenylbenzene1,2-diamine and 2-hydroxy-5-methoxybenzaldehyde at 20 h. The structural properties of compound 3 were characterized in detail by1H NMR,13C NMR, and IR. The cytotoxic activity of the synthesized molecule was investigated on breast cancer cell lines (MDA-MB-231 and MCF-7) and a healthy human embryonic kidney cell line (HEK-293T). The evaluations revealed that the compound exhibits cytotoxic activity against breast cancer cells (IC50: 22.73 µM for MDA-MB-231 and 51.59 µM for MCF-7). Additionally, analyses on healthy cells revealed that the compound exhibits a high selectivity effect (IC50: 352.40 µM). The ADME and toxicity (ADMET) properties of the molecule 3 were analyzed using in silico approaches; physicochemical parameters, lipophilicity, and solubility predictions were calculated using SwissADME, while acute toxicity predictions were obtained through the Protox-II platform. Acute toxicity predictions were reported using Protox-II, and the estimated toxic dose was reported as 563 mg/kg based on the value specified in the study. The GHS toxicity class assigned by Protox-II is 4, which indicates moderate acute toxicity.