Apoptotic Effect of Boric Acid on Human Colon Cancer Cell Line SW-480: An In Vitro Study.


Sevimli M. , Bayram D. , Özgöçmen M. , Armağan İ.

1st International Health Sciences and Life Congress, Burdur, Turkey, 2 - 05 May 2018, pp.19

  • Publication Type: Conference Paper / Summary Text
  • City: Burdur
  • Country: Turkey
  • Page Numbers: pp.19

Abstract

Colon cancer is the third most common cancer worldwide and has high mortality and morbidity rates. The first treatment option is surgery, followed by chemotherapy and radiotherapy. But it has poor prognosis and high recurrence rates. Therefore, it is necessary to develop new and effective treatment approaches. Boric Acid (BA) is the most common form of the “boron” in animals and is a part of a daily diet. Boric acid has some important chemical properties that support its use in cancer therapy. It has been shown that boric acid has antiproliferative and apoptotic properties in some cancer cell lines. In this study we aimed to investigate the apoptotic effects of BA on sw480 human colon cancer cells in monolayer culture conditions. Cells were exposed to BA for 24,48 and 72 hours. TUNEL assay was used to determine apoptotic cells and the proliferation status of cells were monitored with BrdU labelling index. Also to confirm apoptotic processes we used active caspase 3 and AIF immunohistochemical stainings. The IC50 inhibition dose of BA on sw480 cells was 75 mM. After treatment with BA we detect TUNEL positive cells and the number of the dead cells was counted more than the control group. While control group had normal S- phase fraction, only few of BA treated cells nuclei were labelled with BrdU. We observed positive findings for both active caspase 3 and AIF in immunohistochemical stainings.In the light of these results we concluded that BA inhibit the proliferation of sw480 colon cancer cells and induces apoptosis. BA, which is already part of the daily diet and which can be regarded as relatively safe in terms of toxic effects; is an important potential anti-cancer agent.