Extremely high boron tolerance in Puccinellia distans (Jacq.) Parl. related to root boron exclusion and a well-regulated antioxidant system

Hamurcu M., Hakki E. E., Sert T., Ozdemir C., Minareci E., Avsaroglu Z. Z., ...More

ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES, vol.71, no.7-8, pp.273-285, 2016 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 71 Issue: 7-8
  • Publication Date: 2016
  • Doi Number: 10.1515/znc-2015-0226
  • Journal Name: ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.273-285
  • Keywords: antioxidant enzymes, boron toxicity, Puccinellia, reactive oxygen species, root and leaf anatomy, OXIDATIVE STRESS, GYPSOPHILA-ARROSTIL, TOXICITY TOLERANCE, PLANTS, BARLEY, WHEAT, SOIL, MECHANISMS, RESPONSES, CHLOROPLASTS
  • Süleyman Demirel University Affiliated: No

Abstract

Recent studies indicate an extremely high level of tolerance to boron (B) toxicity in Puccinellia distans (Jacq.) Parl. but the mechanistic basis is not known. Puccinellia distans was exposed to B concentrations of up to 1000 mg B L-1 and root B uptake, growth parameters, B and N contents, H2O2 accumulation and center dot OH-scavenging activity were measured. Antioxidant enzyme activities including superoxide dismutase (SOD), ascorbate peroxidase, catalase, peroxidase and glutathione reductase, and lipid peroxidation products were determined. B appears to be actively excluded from roots. Excess B supply caused structural deformations in roots and leaves, H2O2 accumulation and simultaneous up-regulation of the antioxidative system, which prevented lipid peroxidation even at the highest B concentrations. Thus, P. distans has an efficient root B-exclusion capability and, in addition, B tolerance in shoots is achieved by a well-regulated antioxidant defense system.