Cold Plasma Effects on Specific Phenolic Compounds, Soluble Sugar Components and Oxidative Status in Ocimum basilicum L. Callus Cultures
PLASMA CHEMISTRY AND PLASMA PROCESSING, cilt.46, sa.6, ss.108, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 46 Sayı: 6
- Basım Tarihi: 2026
- Doi Numarası: 10.1007/s11090-026-10708-x
- Dergi Adı: PLASMA CHEMISTRY AND PLASMA PROCESSING
- Derginin Tarandığı İndeksler: Applied Science & Technology Source, Engineering Source (EBSCO), Scopus, Materials Science & Engineering Collection (ProQuest), Technology Collection (ProQuest), Aerospace Database, Science Citation Index Expanded (SCI-EXPANDED), Chemical Abstracts Core, Compendex, INSPEC
- Sayfa Sayıları: ss.108
- Süleyman Demirel Üniversitesi Adresli: Evet
Özet
Cold plasma (CP) has attracted growing interest as a residue-free physical elicitation tool in plant biotechnology, yet its effects on targeted phenolic compounds, soluble sugar components, and oxidative markers in plant tissue cultures remain insufficiently characterized. In the present study, we investigated the biochemical responses of Ocimum basilicum L. (basil) callus cultures to a single cold plasma exposure for 0, 60, 120, or 180 s. Specific phenolic compounds (gallic acid, caffeic acid, ferulic acid, catechin, and kaempferol) and soluble sugar components (sucrose, glucose, and fructose) were quantified by HPLC, while hydrogen peroxide (H₂O₂) and malondialdehyde (MDA) were determined spectrophotometrically. CP significantly increased the amount of the specific phenolic compounds, with the strongest response at 120 s, where the total content of the five measured phenolics was 84% higher than in the control. Individual phenolic levels also showed treatment-dependent increases, most of which peaked at 120 s. In contrast, soluble sugar components displayed a biphasic pattern: 60 s CP reduced the individual sugar components and the total value of the quantified soluble sugars, whereas 120 s markedly increased sucrose and restored the total value of the quantified soluble sugars to above-control levels. H₂O₂ accumulated transiently, reaching a maximum at 60 s and returning to basal levels at 180 s, while MDA decreased at 60 s and showed moderate increases at 120 to 180 s, consistent with a controlled rather than severely damaging oxidative response. Multivariate analyses supported a clear treatment-dependent separation of biochemical profiles, with 120 s associated mainly with phenolic enrichment and sucrose accumulation. Overall, the findings indicate that CP can modulate selected biochemical markers in basil callus cultures in a time-dependent manner, and that 120 s represents the most effective exposure among the tested conditions for enhancing the accumulation of the quantified phenolic compounds under the present experimental setup.