Genetic variation among different 29 root-knot nematode populations was characterized by using amplified fragment length polymorphisms (AFLP). Twenty-two nematode samples collected from infected vegetables (tomato, cucumber, eggplant, pepper and melon) at various locations of the. Mediterranean Region in Turkey were screened together with seven reference populations including Meloidogyne arenaria, M. javanica and M. incognita. Genetic polymorphism differentiation of Meloidogyne species was determined Using 11 AFLP primer combinations. Depending oil the primer combination and the genotypes, single lanes with 15-45 amplified DNA fragments were produced. Furthermore, genetic intra-specific similarity of M. arenaria M. incognita, and M. javanica ranged from 40 to 67%, 45 to 56916 and from 41 to 73916, respectively. These findings showed an absence of general correlation between genetic similarity and geographical origin of the populations within species, which was demonstrated using the AFLP patterns.