Benzyl isothiocyanate-loaded chitosan beads: a novel strategy to combat biofilm formation by Staphylococcus aureus and Escherichia coli
Future Microbiology, cilt.21, sa.9, ss.827-839, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 21 Sayı: 9
- Basım Tarihi: 2026
- Doi Numarası: 10.1080/17460913.2026.2688706
- Dergi Adı: Future Microbiology
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Chemical Abstracts Core, EMBASE, MEDLINE, Natural Science Collection (ProQuest), Biological Science Database (ProQuest), Health Research Premium Collection (ProQuest), Pharma Collection (ProQuest)
- Sayfa Sayıları: ss.827-839
- Anahtar Kelimeler: antibacterial, Benzyl isothiocyanate, biofilm inhibition, chitosan bead, molecular docking
- Süleyman Demirel Üniversitesi Adresli: Evet
Özet
Aims: This study aimed to develop and evaluate chitosan beads loaded with benzyl isothiocyanate (BITC) as a novel therapeutic strategy to combat biofilm-associated infections caused by Staphylococcus aureus and Escherichia coli. Methods: The beads were fabricated via ionic gelation and characterized using Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and ultraviolet-visible spectrophotometry (UV-Vis). Antibacterial and antibiofilm activities of the optimal formulation BITC-loaded beads (B-Cs2) were assessed. Supporting computational analyses (molecular docking) were carried out; detailed methods and results are provided in the Supplementary Material. Results: B-Cs2 beads (10 mg) exhibited strong antibacterial activity, producing inhibition zones of 34.0 ± 1.0 mm for S. aureus and 24.0 ± 2.0 mm for E. coli. The formulation also significantly inhibited biofilm formation, reducing it by 49.3% in S. aureus and 48.2% in E. coli. Molecular docking predicted potential interactions with key virulence proteins, suggesting that BITC may disrupt distinct virulence pathways; however, further biochemical validation is required to confirm these proposed mechanisms. Conclusion: BITC-loaded chitosan beads function as a dual-action system with potent antibacterial and antibiofilm properties, demonstrating significant potential for applications in clinical therapeutics and food safety.