Lymphocyte DNA damage in sepsis and septic-shock intensive-care patients: Damage is greater in non-intubated patients


KARABACAK P., TOĞAY V. A. , Celik D. A.

MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, vol.879, 2022 (Peer-Reviewed Journal) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 879
  • Publication Date: 2022
  • Doi Number: 10.1016/j.mrgentox.2022.503516
  • Journal Name: MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
  • Journal Indexes: Science Citation Index Expanded, Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, Chemical Abstracts Core, Chimica, EMBASE, Environment Index, Food Science & Technology Abstracts, Greenfile, MEDLINE, Pollution Abstracts, Veterinary Science Database
  • Keywords: Comet Assay, Critically ill patients, Human lymphocyte, ICU, COMET ASSAY, LUNG INJURY, REPAIR, CELLS

Abstract

Sepsis is an excessive host response to infection; septic shock is a more severe clinical condition. We studied 43 sepsis patients, 32 septic-shock patients, and a group of healthy controls. The patients' Sequential Organ Failure Assessment (SOFA) and Acute Physiology and Chronic Health Evaluation (APACHE) 2 score were much higher in the septic-shock group than in the sepsis group. We used the comet assay to measure lymphocyte DNA damage; the damage scores were significantly higher in both the sepsis and the septic-shock groups compared to the healthy controls. There was no statistically significant difference between the sepsis and septic-shock groups. We also compared DNA damage levels of intubated vs. non-intubated patients. DNA damage was significantly higher in non-intubated patients compared to intubated patients, for both the sepsis and the septic-shock groups. Early intubation may be beneficial in non-intubated patients who have high levels of DNA damage.