Dual magnetic field therapy mitigates LPS-induced pancreatic injury by preserving islet structure and endocrine function in rats Dual manyetik alan tedavisi, sıçanlarda LPS ile indüklenen pankreas hasarını, adacık yapısını ve endokrin fonksiyonu koruyarak azaltır
Pamukkale Medical Journal, cilt.18, sa.4, ss.839-849, 2025 (Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 18 Sayı: 4
- Basım Tarihi: 2025
- Doi Numarası: 10.31362/patd.1720986
- Dergi Adı: Pamukkale Medical Journal
- Derginin Tarandığı İndeksler: Scopus, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.839-849
- Anahtar Kelimeler: LPS, pancreas, pulsed magnetic field, radiofrequency, Sepsis
- Süleyman Demirel Üniversitesi Adresli: Evet
Özet
Purpose: Sepsis is a life-threatening systemic inflammatory response that can lead to multiorgan failure, including pancreatic dysfunction. This study aimed to investigate the potential protective effects of radiofrequency (RF) and pulsed magnetic field (PM) therapies against lipopolysaccharide (LPS)-induced pancreatic injury in rats. Materials and methods: Forty female Wistar albino rats were randomly assigned to five groups (n=8 per group): Control, LPS, LPS + RF, LPS + PM, and LPS + PM + RF. LPS was administered intraperitoneally (5 mg/kg), followed by RF (30 minutes) and/or PM (3 hours) exposure. Pancreatic tissues were examined histopathologically and immunohistochemically for insulin, glucagon, and amylin expression. Results: LPS administration resulted in significant histopathological damage, including hyperemia, edema, and inflammatory infiltration, as well as reduced insulin and amylin immunopositivity and increased glucagon expression in islets. Both RF and PM treatments alleviated these changes, with the combined PM+RF application showing the most prominent protective effects, restoring normal histological architecture and hormonal profiles. Conclusion: RF and PM therapies, particularly in combination, exhibit significant protective effects against sepsis-induced pancreatic injury by mitigating inflammation and preserving islet cell function. These findings highlight a novel non-pharmacological approach for supporting pancreatic health in inflammatory conditions.