Phosducin-like proteins in Dictyostelium discoideum: implications for the phosducin family of proteins
EMBO JOURNAL, cilt.22, sa.19, ss.5047-5057, 2003 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 22 Sayı: 19
- Basım Tarihi: 2003
- Doi Numarası: 10.1093/emboj/cdg508
- Dergi Adı: EMBO JOURNAL
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.5047-5057
- Anahtar Kelimeler: chaperone, Dictyostelium, G protein, signal transduction, phosducin, G-BETA-GAMMA, HETEROTRIMERIC G-PROTEINS, SUBUNIT FUNCTION, REGULATOR, COMPLEX, CHEMOTAXIS, TRANSDUCIN, CELLS, PHLP, GRADIENTS
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- Süleyman Demirel Üniversitesi Adresli: Evet
Özet
Retinal phosducin is known to sequester transducin Gbetagamma, thereby modulating transducin activity. Phos ducin is a member of a family of phosducin-like proteins (PhLP) found in eukaryotes. Phylogeny of 33 phosducin-like proteins from metazoa, plants and lower eukaryotes identified three distinct groups named phosducin-I-III. We discovered three phlp genes in Dictyostelium, each encoding a phosducin-like protein of a different group. Disruption of the phlp1 gene strongly impaired G-protein signalling, apparently due to mislocalization of Gbetagamma in phlp1-null cells. GFP-Gbeta and GFP-Ggamma are membrane associated in wild-type cells, but cytosolic in phlp1-null cells. Phlp2 disruption is lethal due to a synchronous collapse of the cells after 16-17 cell divisions. Phlp3 disruptants show no abnormal phenotype. These results establish a role for phosducin-like proteins in facilitating folding, localization or function of proteins, in addition to modulating G-protein signalling.