Rituximab Attenuated Lipopolysaccharide-Induced Oxidative Cytotoxicity, Apoptosis, and Inflammation in the Human Retina Cells via Modulating the TRPM2 Signaling Pathways

Daldal H., NAZIROĞLU M.

OCULAR IMMUNOLOGY AND INFLAMMATION, vol.30, no.6, pp.1315-1328, 2022 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 30 Issue: 6
  • Publication Date: 2022
  • Doi Number: 10.1080/09273948.2022.2075400
  • Journal Name: OCULAR IMMUNOLOGY AND INFLAMMATION
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, CAB Abstracts, CINAHL, EMBASE, MEDLINE, Veterinary Science Database
  • Page Numbers: pp.1315-1328
  • Keywords: Apoptosis, ARPE-19 cells, human retina cells, lipopolysaccharide, oxidative stress, rituximab, TRPM2 channel, PIGMENT EPITHELIUM, CHANNEL, CD20, DEGENERATION, ACTIVATION, EXPRESSION, INDUCTION, STRESS, ENTRY, DEATH
  • Süleyman Demirel University Affiliated: Yes

Abstract

Purpose We investigated the possible protective effects of rituximab (RTX) on LPS-induced oxidant, inflammatory, and apoptotic adverse actions via the inhibition of TRPM2 channel in the adult retinal pigment epithelial-19 (ARPE-19) cells. Methods In the cultured ARPE-19 cells, we induced five main groups as control, RTX (10 mu g/ml), LPS (1 mu g/ml), LPS+RTX, and LPS+TRPM2 blockers (ACA or 2/APB). Results The levels of apoptosis, cell death, mitochondrial free reactive oxygen radicals (mitROS), cytosolic ROS, lipid peroxidation, caspase -3, caspase -8, caspase -9, ADP-ribose-induced TRPM2 current density, TNF-alpha, IL-1 beta, cytosolic free Zn2+, and Ca2+ were increased by LPS, although their levels were diminished by the treatments of RTX and TRPM2 blockers. Conclusions The LPS-induced mitROS, inflammatory cytokine, and apoptosis levels were modulated via TRPM2 inhibition in the human retinal epithelial cells by the RTX treatment. The RTX may be considered as a new therapeutic approach to LPS-induced human retinal epithelial cell injury.