Investigation of the effects of distance from sources on apoptosis, oxidative stress and cytosolic calcium accumulation via TRPV1 channels induced by mobile phones and Wi-Fi in breast cancer cells


Cig B. , NAZIROĞLU M.

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, cilt.1848, ss.2756-2765, 2015 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 1848 Konu: 10
  • Basım Tarihi: 2015
  • Doi Numarası: 10.1016/j.bbamem.2015.02.013
  • Dergi Adı: BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
  • Sayfa Sayıları: ss.2756-2765

Özet

TRPV1 is a Ca2+ permeable channel and gated by noxious heat, oxidative stress and capsaicin (CAP). Some reports have indicated that non-ionized electromagnetic radiation (EMR)-induces heat and oxidative stress effects. We aimed to investigate the effects of distance from sources on calcium signaling, cytosolic ROS production, cell viability, apoptosis, plus caspase-3 and -9 values induced by mobile phones and Wi-Fi in breast cancer cells MCF-7 human breast cancer cell lines were divided into A, B, C and D groups as control, 900, 1800 and 2450 MHz groups, respectively. Cells in Group A were used as control and were kept in cell culture conditions without EMR exposure. Groups B, C and D were exposed to the EMR frequencies at different distances (0 cm, 1 cm, 5 cm, 10 cm, 20 cm and 25 cm) for 1 h before CAP stimulation. The cytosolic ROS production, Ca2+ concentrations, apoptosis, caspase-3 and caspase-9 values were higher in groups B, C and D than in A group at 0 cm, 1 cm and 5 cm distances although cell viability (MTT) values were increased by the distances. There was no statistically significant difference in the values between control, 20 and 25 cm.