Irbesartan has a curative effect on lipopolysaccharide-induced cardiotoxicity by antioxidant and antiapoptotic pathways O irbesartan tem um efeito curativo na cardiotoxicidade induzida por lipopolissacarídeos por vias antioxidantes e antiapoptóticas


TEPEBAŞI M. Y., AŞCI H., COŞAN S., SEVÜK M. A., KARAKUYU N. F., Özmen Ö.

Revista Portuguesa de Cardiologia, vol.42, no.11, pp.895-903, 2023 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 42 Issue: 11
  • Publication Date: 2023
  • Doi Number: 10.1016/j.repc.2023.03.018
  • Journal Name: Revista Portuguesa de Cardiologia
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Directory of Open Access Journals
  • Page Numbers: pp.895-903
  • Keywords: Apoptosis, Cardiotoxicity, Irbesartan, Oxidative stress, Sepsis
  • Süleyman Demirel University Affiliated: Yes

Abstract

Introduction and Objective: Lipopolysaccharide (LPS) has been associated with myocardial inflammation, oxidative stress, apoptosis, and cardiac dysfunction, as well as death by causing sepsis. In this study, we investigated the effect of irbesartan (IRB), an angiotensin receptor antagonist, on cardiotoxicity caused by LPS. Methods: The experiment involved 24 Wistar albino rats divided into three groups of eight: control, LPS (5 mg/kg), and LPS (5 mg/kg)+IRB (3 mg/kg). Parameters including total oxidative status, total antioxidant status, oxidative stress index, and ischemia-modified albumin were measured to assess oxidative stress in heart tissues and serum. Serum CK, CK-MB, and LDH levels were measured spectrophotometrically. RT-qPCR was used to detect the mRNA expression levels of Bcl-2, BAX, p53, caspase-3, and sirtuin 1. Tissues taken from the heart and aorta were examined by immunohistochemistry and histopathology. Results: While there was an increase in the parameters indicating heart damage, oxidative stress, and apoptosis in the group given LPS, there was an improvement in all parameters and heart damage in the group treated with IRB. Conclusion: As a result of our study, we determined that IRB has an ameliorating effect on myocardial damage caused by oxidative stress and apoptosis developed by the LPS-induced sepsis model.