Nowadays, the use of a wireless network as well as all over the world has increased very quickly. Although WLAN system constitute an important part of our lives almost on a daily, even though there is clear evidence of harmful effects on health available. This idea of the present study was exposed to 2.45 GHz electromagnetic field L. rhamnosus studied on the development of bacteria. Bacterial culture is divided into 16 falcon tube with 15 ml of sterile conditions. During the test tubes were divided into the two groups, control groups (n = 8) and test groups (n = 8). 2.45 GHz half-wave dipole antenna is used as the source of the electromagnetic field, 1 watt maximum power density tubes were exposed to radiation.