Research Information System
3. Uluslararası Sağlık Bilimleri ve İnovasyon Kongresi , Ankara, Turkey, 12 - 13 January 2021, pp.76-77
Paracetamol (Acetaminophen; APAP) is one of the most widely used analgesic and
antipyretic agent today. Paracetamol toxicity due to paracetamol overdose is primarily seen in
the liver. In this study, it was aimed to investigate the effects of Resveratrol and Avocado Oil
on paracetamol-induced hepatotoxicity in rats. In our study, using a total of 30 male Wistar
Albino rats weighing between 400 and 550 grams, 5 separate groups (Group I; Control, Group
II; Paracetamol, Group III; Paracetamol + Resveratrol, Group IV; Paracetamol + Avocado oil,
Group 5; Paracetamol + Resveratrol + Avocado oil) were created. Only isotonic sodium
chloride solution was administered by oral gavage to the rats in the control group. Resveratrol
(10 mg/kg) and Avodako oil (200 mg/kg) were administered by oral gavage for 5 days.
Paracetamol was administered intraperitoneally at a dose of 600 mg/kg on the 4th day.
Animals were sacrificed at 24th hour following paracetamol administration. In our study,
Hematoxylin & Eosin staining was performed on liver tissues taken from rats and liver
damage was evaluated under light microscope. In the control group (Group I), no findings
other than normal histological structures were found in the histological examination of liver
tissue sections. When the liver tissues of the experimental group given paracetamol (Group II)
were examined, compared to the control group; Granular and vacuolar degenerations in
hepatocytes, pycnotic nuclei, mononuclear cell infiltrations in the portal area and parenchyma,
hemorrhagic areas, sinusoidal dilatations, vascular congestions, macro and micro vesicular fat
deposits were observed (p <0.05). In the group treated with Paracetamol and Resveratrol
(Group III), an improvement was observed in histopathological findings compared to the
group given Paracetamol (Group II). In the group that received Avocado with Paracetamol
(Group IV), an improvement in histopathological findings was observed compared to the
group given Paracetamol (Group II), but this improvement was less than the group that
received Resveratrol (group III). Paracetamol, Resveratrol and Avocado were given together
in the group (Group V) compared to the group (Group II) given paracetamol, an improvement
was observed in histopathological findings and this improvement was higher than in group III
and group IV. The results indicated that the combined use of avocado oil, which is rich in oleic acid, having antioxidant properties, known as a healthy fat store, and Resveratrol, a
powerful antioxidant, exhibits protective effects on high dose paracetamol-induced liver
injury compared to their use alone.
Keywords: Paracetamol, Hepatotoxicity, Resveratrol, Avocado oil, Liver injury