In this study, the antimicrobial effect of low concentrations of nisin, carvacrol and EDTA alone and their combinations against S. Typhimurium SL1344 in TSBYE at 4 degrees C and 37 degrees C for 24 h were investigated. Carvacrol was found the most effective chemical among the single antimicrobial treatments. Carvacrol at 0.5 or 1 mM plus EDTA at 2 mM showed a synergistic effect at 37 degrees C. This effect was increased when they were combined with nisin both at 4 degrees C and 37 degrees C. The most effective application was determined the combinations of nisin at 400 IU/ mL, carvacrol at 1 mM and EDTA at 2 mM. This combination reduced S. Typhimurium counts to undetectable level after 12 and 6 h of incubation at 4 degrees C and 37 degrees C, respectively. The results of this study indicated that combinations of low concentrations of nisin, carvacrol and EDTA could potentially be used as food preservative against S. Typhimurium, to provide better quality foods.