Fatty acids containing stearic acid. which are found in hydrogenated fat, may have a detrimental effect oil the cholesterol and triacylglycerol (TAG) content of plasma lipoproteins, and oil the absorption of fatty acids and fat-soluble vitamins. The aim of our study was to examine the tissue concentration of lipids and vitamins A and E after feeding a hydrogenated soybean on (HSO) diet to rats. Twenty male Sprague-Dawley rats were randomly divided into two groups. fed on Coconut Oil (control) and HSO. respectively in amounts corresponding to 15% of the total feed. Plasma total cholesterol. VLDL- and LDL-cholesterol, lipid peroxidation and daily excretion of the TAG and cholesterol in feces were higher in the HSO than in the control group. TAG values in plasma and liver. and HDL-cholesterol levels in plasma were lower in the HSO than in the control group. The same was true for phospholipids in plasma and for saturated fatty acids. mono- and polyunsaturated fatty acids levels in the liver and vitamin E in plasma, LDL and adipose tissue. The results of this study provide new evidence concerning the effect of dietary hydrogenated fat oil lipid. TAG and vitamin E Status, which are important for maintenance of good health. Consumption of dietary HSO may be associated with cardiovascular disease.