The current study is to determine variation in vitro antioxidant activities and chemical contents of different extraction solvents in aerial parts of wild M. longifolia subsp. typhoides var. typhoides taxons from Tokat, Turkey. The chemical and volatile compounds of M. longifolia were investigated with HPLC-TOF/MS and GC/MS. The highest total phenolic and flavonoid contents were found as 28.27 and 25.42 mg from R6W, respectively. Hesperidin, neohesperidin, taxifolin, fumaric acid, chlorogenic acid, naringin, 4-hydroxybenzoic acid, caffeic acid, protocatechuic acid and syringic acid were determined in all extracts. The linalool, menthone, isomenthone, piperitone oxide, pulegone, thymol, caryophyllene and caryophyllene oxide were major components in volatile compounds. The highest activities were observed significantly (p < 0.01) from R6M, R6W, R10EA and R5M in total antioxidant, superoxide-scavenging, metal-chelating and inhibition of lipid peroxidation activities, respectively. The isolated menthone (96.00%) was exhibited the higher inhibition of lipid peroxidation than standards. The results validate that M. longifolia possesses as a source of antioxidant potential for medicinal and foods.