5th International Congress on Cell Membranes and Oxidative Stress:Focus on Calcium Signaling and TRP Channels, Isparta, Turkey, 9 - 12 September 2014, vol.6, pp.394
Mistletoe ( Viscum album L., VA), a semiparasitic
plant of the Loranthacea family, grows on deciduous
trees like the apple, oak, or on coniferous tress like
pine and fir. It has been used in tradional medicine
as a sedative, vasodilator, diuretic, analgesic, antispasmolytic, cardiotonic and anticancer agent.
Commercially available extracts of VA, including
Iscador (Iscar), Eurixor, Helixor, Isorel (Vysorel),
Iscucin, Plenosol (Lektinol), are used often the
protocols of adjuvant treatment with standart
chemotherapy or radioterapy agent because of
their immunomodulatory and cytotoxic properties
This work aimed to study the apoptotic effects of
the mistletoe extracts Helixor A (HA), Helixor P (HP)
and Helixor M (HM) on human breast cancer cell line
MDA-MB-231 using Poly-ADP-Ribose-Polimerase
(PARP) staining and Terminal deoxynucleotidyl
transferase dUTP nick end labeling (TUNEL) assay.
Materials and Methods
MDA-MB-231 cell line was cultured in monolayer
model. Cells were treated with the mistletoe extracts
HA, HM and HP on 24, 48 and 72 hours incubation.
Cells were not treated with the mistletoe extracts
were considered as the control group. The effects
of the extracts effective doses on PARP and Tunel
staining were assessed by immunohistochemically.
IC50 values of HA and HP in MDA-MB-231 are
500μg/ml, 50μg/ml and 5μg/ml on 24, 48 and 72
hours incubation respectively. IC50 values of HM
in MDA-MB-231 are 500μg/ml on all incubations.
PARP staining and Tunel assay was used together
to determine the death of the cells. TUNEL positive
cells and active PARP were detected after treatment
in monolayer model. Dead cell count was more in
the mistletoe extracts HA, HM and HP applied MDAMB-231 cell lines in comparison to the controls (p
In this study, mistletoe extracts HA, HM and HP
applications enhanced the TUNEL positive cells
and active PARP in comparison to the controls in