The viscum Album L. Extracts Induce Apoptosis Of Human Breast Cancer Mda-Mb-231 Cells


Sakallı Çetin E., Bayram D., Kara M., Candan İ. A. , Özgöçmen M.

5th International Congress on Cell Membranes and Oxidative Stress:Focus on Calcium Signaling and TRP Channels, Isparta, Turkey, 9 - 12 September 2014, vol.6, pp.394

  • Publication Type: Conference Paper / Summary Text
  • Volume: 6
  • City: Isparta
  • Country: Turkey
  • Page Numbers: pp.394

Abstract

Aim Mistletoe ( Viscum album L., VA), a semiparasitic plant of the Loranthacea family, grows on deciduous trees like the apple, oak, or on coniferous tress like pine and fir. It has been used in tradional medicine as a sedative, vasodilator, diuretic, analgesic, antispasmolytic, cardiotonic and anticancer agent. Commercially available extracts of VA, including Iscador (Iscar), Eurixor, Helixor, Isorel (Vysorel), Iscucin, Plenosol (Lektinol), are used often the protocols of adjuvant treatment with standart chemotherapy or radioterapy agent because of their immunomodulatory and cytotoxic properties This work aimed to study the apoptotic effects of the mistletoe extracts Helixor A (HA), Helixor P (HP) and Helixor M (HM) on human breast cancer cell line MDA-MB-231 using Poly-ADP-Ribose-Polimerase (PARP) staining and Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. Materials and Methods MDA-MB-231 cell line was cultured in monolayer model. Cells were treated with the mistletoe extracts HA, HM and HP on 24, 48 and 72 hours incubation. Cells were not treated with the mistletoe extracts were considered as the control group. The effects of the extracts effective doses on PARP and Tunel staining were assessed by immunohistochemically. Results IC50 values of HA and HP in MDA-MB-231 are 500μg/ml, 50μg/ml and 5μg/ml on 24, 48 and 72 hours incubation respectively. IC50 values of HM in MDA-MB-231 are 500μg/ml on all incubations. PARP staining and Tunel assay was used together to determine the death of the cells. TUNEL positive cells and active PARP were detected after treatment in monolayer model. Dead cell count was more in the mistletoe extracts HA, HM and HP applied MDAMB-231 cell lines in comparison to the controls (p <0.05). Conclusion In this study, mistletoe extracts HA, HM and HP applications enhanced the TUNEL positive cells and active PARP in comparison to the controls in monolayer model