The objective of this study was to determine the relationships between nitrogen (N), phosphorus ( P), potassium ( P), acid detergent fiber (ADF) and neutral detergent fiber (NDF) contents of sainfoin (Onobrychis sativa Lam.) pasture and canopy reflectance. Canopy reflectance measurements were made by using a portable spectroradiometer. An experiment was conducted in the Turkey in May and June in 2007 and 2008. Sainfoin pasture N, P, K, ADF and NDF contents correlated linearly with the reflectance ratios R780/650 (0.61 <= r(2) <= 0.80) and first derivatives of the reflectance ratios 760/630 (0.70 <= r(2) <= 0.84). Linear equations between each forage variable and reflectance or first derivatives reflectance had high r(2) (0.68 <= r(2) <= 0.83 and 0.79 <= r(2) <= 0.90, respectively) in R780 and R760 wavelengths. In stepwise regression of the reflectance ( in 460, 550, 650 and 780 nm wavelengths), the r(2) of predicted and measured N, P, K, ADF and NDF contents of sainfoin pasture were (0.85, 0.85, 0.78, 0.81 and 0.74, respectively), in stepwise regression of the first derivatives of reflectance (in 440, 530, 630 and 760 nm wavelengths), the r(2) of predicted and measured N, P, K, ADF and NDF contents of sainfoin pasture were (0.87, 0.91, 0.83, 0.93 and 0.86, respectively). Our results suggest that canopy reflectance in blue, green, red and near infrared wavebands with NIR/Red and NDVI ratios can be used for nondestructive prediction of forage quality variables in sainfoin pasture.