Commercial bumblebee colonies have been used for the pollination of a number of crops, mainly of tomatoes (95%). In comparison with other bumblebee species, Bombus terrestris has the most year round rearing. The year round rearing procedure of B. terrestris involves some main stages: colony foundation, obtaining of young queens and males, mating and diapause control. Success of these stage are affected by environmental conditions, physiological properties and genetic structure of the queens. Knowledge of the effects of these factors will increase the rearing success. Because of the B. terrestris queens mate once, female individuals in their colonies are supersister. There is a 75% genetic relatedness between the supersister young queens. This study was conducted to compare the different super sister queen groups and to determine the effect of genotype on weight, mating performance and diapause performance of queensin B. terestris. Results showed that genetic differences affected the copulation duration and pre and post diapause weight of queens, but survival ratio during the diapause duration did not affected by genotypic differences.