Effects Of Non-steroid Antiinflammatory Drugs On Adhesion Molecules Of Smooth Muscle Cells (non-steroid Antiinflamatuar Ilaçların Çizgili Kas Dokusundaki Adhezyon Molekülleri Üzerine Etkisi)


Candan İ. A. , Öncü M. , Özgöçmen M. , Darıcı H.

11th National Histology And Embryology Congress, Cell And Tissue Biology Research, Denizli, Turkey, 16 - 19 May 2012, pp.197-198

  • Publication Type: Conference Paper / Summary Text
  • City: Denizli
  • Country: Turkey
  • Page Numbers: pp.197-198

Abstract

Role of adhesion molecules in inflammation is crucial. Vascular cell adhesion molecule-1 (VCAM-1), an Ig superfamily member, found in many cell types, expressed especially in inflammated regions. Another molecule related with inflammation is nitric oxide (NO) which is synthesized by nitric oxide sytnhase (NOS) enzyme. Long term use of some non-steroid anti-inflammatory drugs (NSAID) such as methamizol and diclofenac causes continuous increase in NOS levels thereby negative effects of NO. We aimed to investigate the effects of long term usage of NSAIDs on VCAM-1 and a NOS isoform inducible NOS (iNOS) in skeletal muscle. We also investigated whether the inhibition of iNOS by L-NAME has an effect on VCAM-1 expression. 40 Swiss male mice were divided into four groups. Intraperitoneal injections were made as 0,1 ml of saline to control group (Group I), 10 mg/ml methamizol sodium to group II, 20 mg/ml diclofenac potassium to group III and 20 mg/ml L-NAME to group IV once a day for 10 days. Skeletal muscle tissues removed from legs and fixed. Immunohistochemical staining with VCAM-1 and iNOS antibodies was performed using ABC method. Slides were observed under light microscope and evaluated by staining location and intensity. Mann-Whitney U and Kruskal-Wallis tests were performed via SPSS 15.0. VCAM-1 and iNOS staining were observed in capillaries and medium sized arteries and veins. But no statistically meaningful difference was found when immunohistochemical staining intensity was evaluated between experiment and control groups. Literature about the effects of NSAIDs on adhesion molecules is very limited. Although our results showed no effects of NSAIDs on VCAM-1 expression levels in endothelial cells of skeletal muscle, we think different results can be obtained in further studies with longer exposure periods or higher doses. Keywords: Striated muscle, Cell adhesion molecules, Nonsteroidal antiinflammatory agents, Immunohistochemistry.