Investigating the protective effects of fluvoxamine against sepsis-related acute lung injury through antiapoptotic, antiinflammatory, and anti-oxidant features in rats
Iranian Journal of Basic Medical Sciences, cilt.28, sa.3, ss.323-331, 2025 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 28 Sayı: 3
- Basım Tarihi: 2025
- Doi Numarası: 10.22038/ijbms.2024.80608.17444
- Dergi Adı: Iranian Journal of Basic Medical Sciences
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, Index Islamicus, Veterinary Science Database, Directory of Open Access Journals
- Sayfa Sayıları: ss.323-331
- Anahtar Kelimeler: ER stress, Inflammation, Lipopolysaccharide, Rat, Respiratory system, SSRI
- Süleyman Demirel Üniversitesi Adresli: Evet
Özet
Objective(s): Acute lung injury (ALI) is characterized by severe hypoxia and alveolar damage, often caused by oxidative stress, endoplasmic reticulum stress (ERS), and apoptosis. Fluvoxamine (FLV), an antidepressant, has tissue-protective properties through various intracellular mechanisms. This study investigates the anti-inflammatory effects of FLV used as an antidepressant in a lipopolysaccharide (LPS)-induced ALI model. Materials and Methods: Thirty-two female Wistar Albino rats aged 14-16 weeks and weighing 300- 350 g, with 8 animals in each group, were divided into four groups: control, LPS, LPS+FLV, and FLV. After LPS administration, rats were euthanized, and histopathological analysis, immunohistochemistry for tumor necrosis factor-α (TNF-α) and caspase-3 (Cas-3), ELISA for oxidative stress markers, and PCR for CHOP, Cas-12, and Cas-9 gene expressions were conducted. Results: In the LPS group, lung tissue damage, increased inflammatory cell infiltration, increased Cas- 3 and TNF-α expressions, increased oxidative stress markers, and increased CHOP, Cas-9, and Cas- 12 mRNA expressions were observed compared to the control group. FLV treatment in the LPS+FLV group significantly reversed these effects in the LPS group. Conclusion: FLV exhibits protective effects against ALI by mitigating inflammation, ERS, and apoptosis via the CHOP/Cas-9/Cas-12 pathway. Further studies are needed to explore additional pathways and potential clinical applications of FLV.