The aim of this study was to compare the clinical efficacy and side effects of a new daytime at-home bleaching technique (28% carbamide peroxide [CP] gel) with the overnight application of 10% CP and to determine the change of tooth color 1-year post-treatment. Twenty healthy volunteers were assigned to one of two sample groups of 10. All participants employed one of the two at-home bleaching systems: (1) the new daytime at-home bleaching system including 28% CP gel with a non-custom-fit tray (Meta Tray, Remedent, Deurle, Belgium) for 20 minutes, and (b) the conventional overnight at-home bleaching system with a 10% CP gel and a custom-fit tray (Opalescence PF, Ultradent, South Jordan, UT, USA) for 6 to 8 hours. Digital images and CIE (International Commission on Illumination) L*, a*, and b* spectrophotometric measurements were taken at baseline, after the bleaching treatment and 1-year post-treatment. Tooth and gingival sensitivity was measured with a specially designed 4-point scale. Significant differences were found in L*, a*, and b* values, between initial and post-treatment, for both bleaching systems (p < 0.05). However, no significant difference was detected between post-treatment and 1-year follow-up. The bleaching effectiveness of Opalescence PF was found to be superior to that of Meta Tray, considering the color parameter of Delta E (p < 0.05). Meta Tray provoked less tooth sensitivity (p < 0.05), however gingival sensitivity appeared more in this group (p < 0.05). Within the limitations of this study, it can be concluded that the new daytime at-home bleaching system tested (Meta Tray) produced significant bleaching effects. However, the clinical efficacy of the overnight bleaching system was found to be superior to the daytime at-home bleaching system evaluated in this study. The whitening effect remained similar 1-year after the bleaching treatment for both at-home bleaching systems.