Melatonin Metabolites Protect Human Retinal Pigment Epithelial Cells from Death Caused by Oxidative Stress


Ebru U., Derya K., Hakan K. A. , Bilge A., Adem D.

PHARMACEUTICAL CHEMISTRY JOURNAL, vol.55, no.8, pp.762-768, 2021 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 55 Issue: 8
  • Publication Date: 2021
  • Doi Number: 10.1007/s11094-021-02490-1
  • Title of Journal : PHARMACEUTICAL CHEMISTRY JOURNAL
  • Page Numbers: pp.762-768
  • Keywords: 6-hydroxy melatonin, N-acetyl serotonin, oxidative stress, retinal pigment epithelium, GAMMA-AMINOBUTYRIC-ACID, N-ACETYLSEROTONIN, ANTIOXIDANT, DAMAGE, 6-HYDROXYMELATONIN, PROGABIDE, RECEPTOR

Abstract

This study was aimed to determine the efficacy of 6-hydroxy melatonin and N-acetyl serotoninin protecting human retinal pigment epithelial cells from death brought about by oxidative-stress. The ARPE-19 cells (one of the retinal pigment epithelial cell lines) were used as the test cell culture. Following the application of 500 mu M H2O2, ARPE-19 cell line culture was incubated for 20 h to induce oxidative stress damage. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide) assay was used to evaluate the oxidative injury to ARPE-19 cells caused by H2O2 and the effectiveness of 6-hydroxy melatonin and N-acetyl serotonin application in preventing this damage. The results indicated that 6-hydroxy melatonin and N-acetyl serotonin exhibited a therapeutic effect on cells damaged by exposure to oxidative stress. It was concluded that these melatonin metabolites might be useful in treating many retinal conditions linked to the oxidative stress-induced damage. In addition, this study proposed a new idea for developing chemical approaches to the design and synthesis/semisynthesis of new drugs.