Melatonin Metabolites Protect Human Retinal Pigment Epithelial Cells from Death Caused by Oxidative Stress

Ebru U., Derya K., Hakan K. A., Bilge A., Adem D.

PHARMACEUTICAL CHEMISTRY JOURNAL, vol.55, no.8, pp.762-768, 2021 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 55 Issue: 8
  • Publication Date: 2021
  • Doi Number: 10.1007/s11094-021-02490-1
  • Journal Name: PHARMACEUTICAL CHEMISTRY JOURNAL
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, Biotechnology Research Abstracts, Chemical Abstracts Core, EMBASE
  • Page Numbers: pp.762-768
  • Keywords: 6-hydroxy melatonin, N-acetyl serotonin, oxidative stress, retinal pigment epithelium, GAMMA-AMINOBUTYRIC-ACID, N-ACETYLSEROTONIN, ANTIOXIDANT, DAMAGE, 6-HYDROXYMELATONIN, PROGABIDE, RECEPTOR
  • Süleyman Demirel University Affiliated: Yes

Abstract

This study was aimed to determine the efficacy of 6-hydroxy melatonin and N-acetyl serotoninin protecting human retinal pigment epithelial cells from death brought about by oxidative-stress. The ARPE-19 cells (one of the retinal pigment epithelial cell lines) were used as the test cell culture. Following the application of 500 mu M H2O2, ARPE-19 cell line culture was incubated for 20 h to induce oxidative stress damage. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide) assay was used to evaluate the oxidative injury to ARPE-19 cells caused by H2O2 and the effectiveness of 6-hydroxy melatonin and N-acetyl serotonin application in preventing this damage. The results indicated that 6-hydroxy melatonin and N-acetyl serotonin exhibited a therapeutic effect on cells damaged by exposure to oxidative stress. It was concluded that these melatonin metabolites might be useful in treating many retinal conditions linked to the oxidative stress-induced damage. In addition, this study proposed a new idea for developing chemical approaches to the design and synthesis/semisynthesis of new drugs.