Effects of Tc-99m sestamibi on antioxidant defense system and lipid peroxidation in the heart of Sprague Dawley rats

CESUR G., KUMBUL DOĞUÇ D., YILDIZ M., Ogut S., Polat M., Ongel K.

TOXICOLOGY AND INDUSTRIAL HEALTH, vol.30, no.2, pp.154-159, 2014 (Peer-Reviewed Journal) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 30 Issue: 2
  • Publication Date: 2014
  • Doi Number: 10.1177/0748233712452599
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.154-159


Nuclear medicine has been using radiopharmaceuticals for the diagnostic and therapeutic purposes of many diseases. Technetium-(99m) methoxyisobutylisonitrile (Tc-99m sestamibi) is a lypophilic complex that has a positive-loaded isonitril group. Aim of the study is to investigate whether Tc-99m sestamibi, which is one of the mostly used radiopharmaceuticals in nuclear medicine field, causes oxidative damage or not in rats' heart after an injection. A total of 16 male Sprague Dawley rats were randomly divided into two groups: group I: Tc-99m sestamibi group, Tc-99m sestamibi administered intravenously with the dose of 25MBq; group II: control group, one dose of isotonic sodium chloride was administered intravenous with the same volume as Tc-99m sestamibi group. Malondialdehyde (MDA) and total oxidant status (TOS) were used as markers of oxidative stress-induced heart impairment. Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and total antioxidant status (TAS) activities were studied to evaluate the changes in the antioxidant status. In the Tc-99m sestamibi group (group I), animals treated with Tc-99m sestamibi produced a significant decrease in the activities of antioxidant enzymes (SOD and CAT), while MDA level increased when compared with control group (group II) in myocardial tissue (p<0.05). On the other hand, the GSH-Px activities were significantly increased in the Tc-99m sestamibi-treated rats compared with the untreated rats (p<0.05). There was no significant difference in the TAS and TOS levels of plasma.