Antioxidant and Enzyme Inhibitory Properties, and HPLC-MS/MS Profiles of Different Extracts of Arabis carduchorum Boiss.: An Endemic Plant to Turkey

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Uba A. I. , Zengin G., Montesano D., ÇAKILCIOĞLU U., SELVİ S., Ulusan M. D. , ...More

APPLIED SCIENCES-BASEL, vol.12, no.13, 2022 (Peer-Reviewed Journal) identifier identifier

  • Publication Type: Article / Article
  • Volume: 12 Issue: 13
  • Publication Date: 2022
  • Doi Number: 10.3390/app12136561
  • Journal Indexes: Science Citation Index Expanded, Scopus, Aerospace Database, Agricultural & Environmental Science Database, Applied Science & Technology Source, Communication Abstracts, INSPEC, Metadex, Directory of Open Access Journals, Civil Engineering Abstracts
  • Keywords: Arabis carduchorum, phenolics, flavonoids, HPLC-MS, MS, solvent polarity, antioxidant, enzyme inhibition, ACCUMULATION, ALPINA, SEEDS


In this study, six extracts of Arabis carduchorum prepared from solvents of varying polarity (water, methanol, ethyl acetate, dichloromethane, and n-hexane) were investigated for their chemical profiles and total phenolic and flavonoid contents (TPC/TFC) using HPLC-MS/MS and spectrophotometric/colorimetric assays, respectively, along with the assessment of their antioxidant and enzyme inhibitory effects. In general, the polar extracts (methanol, water, and infusion) showed higher TPC/TFC than the other extracts (range: 13.73-26.62 mg GAE/g; 2.66-52.95 mg RE/g, respectively). The total antioxidant capacity of the polar extracts ranged from 0.90-1.85 mmol TE/g in phosphomolybdenum assay, while the free radical scavenging potential ranged from 4.36-32.13 mg TE/g and 12.58-67.73 mg TE/g in DPPH and ABTS assays, respectively. While the water and infuse extract inhibited only butyrylcholinesterase (2.68 and 2.39 mg GALAE/g, respectively), the other extracts were found to inhibit both acetyl- and butyryl-cholinesterases (0.96-2.73 mg GALAE/g and 1.86-5.11 mg GALAE/g, respectively). On the contrary, all extracts exhibited anti-tyrosinase (40.24-59.22 mg KAE/g) and anti-amylase (0.09-0.63 mmol ACAE/g) activities. Overall, this study highlighted the effect of solvent polarity on the extraction of bioactive compounds and the biological properties of the extracts of A. carduchorum; hence, the results obtained could be used to guide optimum extraction in future studies.