Lignocellulosics fibers and commercially-manufactured-chip (Pinus sylvestris L., Fagus orientalis and Quercus robur L.) with 11% moisture conten twere used for the experiment. The mixingratios of lignocellulosics fibers was 20% which is from okra and tobaccos talks, hazelnut and walnuts hell, and pinecone for each mixture in preformed panel and commercially manufactured-chip was 100 % for the control sample. A commercial ureaformaldehyde (UF) adhesive was used as a binder. The physical and mechanical properties such as density, thickness swelling (TS), bending strength (BS), modulus elasticity (MOE), internalbond (IB), screw holding ability (SHA) perpendicular to the plane of panel, Janka hardness perpendicular to the plane of panel properties of MDF were measured. The results indicated that all the panels met the general purpose-use requirements of TS-EN. Thus, our results suggest that biomass from different sources can be an alternative raw material for MDF manufacturing process.