Effects of different temperatures (7, 15, 25 and 30 degrees C) on in vitro pollen germination and pollen tube growth were evaluated in six strawberry cultivars, including 'Allstar', 'Cavendish', 'Chandler', 'Elsanta', 'Elvira' and 'Selva'. Except for 'Elsanta', the viabilities of pollen from all cultivars were found considerably high in 2, 3, 5-triphenly tetrazolium chloride (TTC) stain test. Temperature has a significant effect on pollen germination and pollen tube growth. The highest pollen germination occurred at 30 degrees C for 'Chandler' and 'Selva' but at 25 degrees C for 'Elsanta', 'Cavendish', 'Allstar' and 'Elvira'. A longer incubation period increased the rate of in vitro pollen germination and all cultivars showed the highest germination percentage after 24 hours of incubation. In vitro elongation of pollen tubes was clearly affected by incubation temperature, and cultivars reached their maximum pollen tube growth at different temperature.