The Effect of a New Class Phytohormone Strigolactone on in vitro Callus Formation of Oregano (Origanum onites L.) Plant


Coşkun Y. , Hudu H.

The Effect of a New Class Phytohormone Strigolactone on in vitro Callus Formation of Oregano (Origanum onites L.) Plant, Burdur, Turkey, 17 - 19 December 2020, pp.80

  • Publication Type: Conference Paper / Summary Text
  • City: Burdur
  • Country: Turkey
  • Page Numbers: pp.80

Abstract

Origanum onites L. is one of the main aromatic species exported from Turkey with medicinal properties. It is known to have antiseptic, antibacterial, antispasmodic, antifungal, antimicrobial, anti-inflammatory and anticarncinogenic properties due to its volatile oils and terpenic materials. Plant production using the tissue culture technique is a method of production that enables medicinal and aromatic plants to be reproduced vegetatively rapidly and in large quantities, as in many plant species. Strigolactones are associated with regulatory roles in plant growth and development, including seed germination, root and shoot architecture patterning, nutrient acquisition, symbiotic interactions, as well as mediation of plant responses to abiotic and biotic cues. In this work, we aimed to evaluate the effect of a new class phytohormone strigolactone on in vitro callus formation of Oregano plant. Callus cultures were obtained by initiating leaf explant on Murashige and Skoog (MS) medium supplemented with 6-Benzylaminopurine (BAP; 2.0 mg l-1) in combination with 1-Naphthalene acetic acid (NAA; 1.0 mg l-1) and various concentrations of strigolactones (0.0, 0.1, 0.5, 1.0, 2.0 and 5.0 µM) were added. In each stage of the experiment, explants placed on MS media with 2.0 mg l-1 BAP and 1.0 mg l-1 NAA without the addition of strigolactone were the control. After 21 days of callus induction, it was found that all the explants produced a compact callus. Furthermore, after 35-40 days during the callus multiplication stage, it was observed that the best mass of the callus tissue was the media supplemented with 2.0 µM strigolactone. It was also observed that the mass of the propagated callus tissue decreased with an increase of strigolactone. The results obtained indicate that a lower concentration of strigolactone has positive impact on callus development. This protocol of ours presents the first of its kind and will lead the way for future studies.