Genotoxic evaluation of Halfenprox using the human peripheral lymphocyte micronucleus assay and the Ames test


Akyil D., Eren Y. , Konuk M., Dere H., Serteser A.

DRUG AND CHEMICAL TOXICOLOGY, vol.40, no.2, pp.191-195, 2017 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 40 Issue: 2
  • Publication Date: 2017
  • Doi Number: 10.1080/01480545.2016.1193865
  • Title of Journal : DRUG AND CHEMICAL TOXICOLOGY
  • Page Numbers: pp.191-195

Abstract

The genotoxicity and mutagenicity of Halfenprox, a synthetic pyrethroid insecticide and acaricide, was assessed using two standard genotoxicity assays of the Salmonella typhimurium mutagenicity assay (Ames test) and in vitro micronucleus (MN) assay in human peripheral lymphocytes. In the Ames test, Salmonella strains TA98 and TA100 were treated with or without S9 fraction. The doses of Halfenprox were 6.25, 12.5, 25, 50, and 100 mg/plate and test materials were dissolved in DMSO. The concentrations of Halfenprox did not show mutagenic activity on both strains with and without S9 fraction. The MN assay was used to investigate the genotoxic effects of Halfenprox in human peripheral lymphocytes treated with 250, 500, 750, and 1000 mg/ml concentrations of Halfenprox for 24 and 48 h, and at 1000 mg/ml the concentration was significantly increased and the MN formation was compared with the negative control for both treatment periods. In addition, a significant decrease of the nuclear devision index (NDI) values at the higher concentrations of Halfenprox and at both treatment periods was observed.