Electrochemical Polymerization of 2-Thiophen-3-yl-malonic Acid for Biosensor Application


Celik G., EREN E. , Uygun A.

JOURNAL OF APPLIED POLYMER SCIENCE, cilt.128, ss.636-641, 2013 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 128 Konu: 1
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1002/app.38205
  • Dergi Adı: JOURNAL OF APPLIED POLYMER SCIENCE
  • Sayfa Sayıları: ss.636-641

Özet

Poly(2-thiophene-3-yl-malonic acid) (P3TMA) was synthesized electrochemically using tetrabutylammonium perchlorate (TBAClO(4)) as electrolyte into acetonitrile (ACN) for glucose biosensor application. The properties of P3TMA were investigated using FTIR, SEM, and electrochemical measurements. The glucose oxidase enzyme (GOD) was chemically immobilized onto P3TMA modified electrode using glutaraldehyde as crosslinking. It was observed that response current of P3TMA/GOD enzyme electrode increased linearly with loading glucose concentration. Michaelis Menten constant (K-m) of P3TMA/GOD enzyme electrode was calculated as 0.03517 mM. FTIR and SEM analyses were used to confirm immobilization of GOD onto surface of P3TMA-modified electrode. (C) 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 128: 636-641, 2013