Thymoquinone reduced RIPK1-dependent apoptosis caused by valproic acid in rat brain
Annals of Medical Research, cilt.28, sa.11, ss.2005-2011, 2021 (TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 28 Sayı: 11
- Basım Tarihi: 2021
- Doi Numarası: 10.5455/annalsmedres.2021.01.046
- Dergi Adı: Annals of Medical Research
- Derginin Tarandığı İndeksler: EBSCO Legal Source, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.2005-2011
- Süleyman Demirel Üniversitesi Adresli: Evet
Özet
Abstract
Aim: Valproic acid (VPA) is a commonly used antiepileptic drug and known to have a neurotoxic effect, but its mechanism is not yet
understood. In the present study, we aimed to determine how the VPA causes cell death in the brain and to evaluate the protective
effects of thymoquinone (TQ) on VPA-induced brain damage.
Materials and Methods: Male Sprague–Dawley albino rats were divided into three groups: control, VPA (500 mg/kg/day) and VPA +
TQ (500 mg/kg/day + 50 mg/kg/day) with seven rats in. At the end of the experiment, rats were sacrificed and brain samples were
taken to measure the expression levels of Receptor-interacting serine/threonine-protein kinase-1 (RIPK1) and -3 (RIPK3) genes by
quantitative real-time PCR (qRT-PCR), NADPH oxidase-4 (NOX4) and, caspase-3 (CAS-3) expression by immunohistochemistry and
the structural changes in the brain tissue by histologically.
Results: RIPK1 gene expression levels were significantly increased in the VPA group compared to the controls (p<0.05) and a
decrease in VPA + TQ group against the VPA group. Also, NOX-4 and CAS-3 production were increased in the VPA group compared
to the control group (p<0.05), and there is a markedly decrease in the VPA + TQ group compared to the VPA group.
Conclusion: VPA induced RIPK1-dependent apoptosis, leading to cell deaths in the brain and TQ reduced its effects. Therefore, TQ
uptake can be a supportive treatment method for long-term and high-dose VPA users to eliminate undesirable effects.
Keywords: Apoptosis; RIPK1; thymoquinone; valproic acid