The volatile component composition of leaves and flowers for Ballota nigra subsp. anatolica P.H. Davis that were collected between 2015 and 2016 from Camlica provice of Kutahya city during three different vegetation periods as pre-bloom, bloom and post flowering were determined by gas chromatography mass spectroscopy (GC-MS) after solid phase micro extraction (SPME). 59 different volatile oil components were determined of Ballota nigra subsp. anatolica. As main components, Hexenal (17.60%), germacrene D (6.70%) and beta-caryophyllene (8.80%) were found during pre-blooming period, also Hexenal (21.16%), germacrene D (7.77%) and beta-caryophyllene (10.03%) in blooming period and Hexenal (15.88%), germacrene D (7.60%) and beta-caryophyllene (9.48%) in post-flowering period.