The aim of this study was to investigate whether individuals with dental fluorosis (DF) have signs and symptoms of temporomandibular joint (TMJ) disorders (TMD). The study group consisted of 71 dental patients with DF who were born and living in an endemic fluorosis region of Isparta, Turkey. The control group comprised 29 patients not having DF, who applied to our clinics from neighboring communities with low levels of F (<0.3 ppm) in the drinking water. DF in the 71 patients of the study group was classified using a modified version of the Thylstrup-Fejerskov Index: 21 mild, 23 moderate, and 27 severe. All 100 participants were examined clinically, and any signs and symptoms of TMJ disorders (TMD) were recorded using a standard evaluation form. Radiological changes in the TMJ were studied using plain lateral TMJ projection. No statistically significant difference in clinical signs and symptoms of TMJ-TMD was detected between individuals with or without DF. There were also no statistically significant differences in radiological changes in TMJ between the two groups. Thus, this small-scale preliminary survey did not reveal an association between DF and TMJ-TMD. Further investigation is needed, however, to determine dose-related effects of high-F intake on the human stomatognathic system.