Intracellular Ca2+ and antioxidant values induced positive effect on fertilisation ratio and oocyte quality of granulosa cells in patients undergoing in vitro fertilisation


Tola E. N. , MUNGAN M. T. , UĞUZ A. C. , NAZIROĞLU M.

REPRODUCTION FERTILITY AND DEVELOPMENT, cilt.25, ss.746-752, 2013 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 25 Konu: 5
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1071/rd12144
  • Dergi Adı: REPRODUCTION FERTILITY AND DEVELOPMENT
  • Sayfa Sayıları: ss.746-752

Özet

Oxidative stress is important for promoting oocyte maturation and ovulation within the follicle through calcium ion (Ca2+) influx. The relationship between antioxidant and cytosolic Ca2+ levels and oocyte quality and fertilisation rate in the granulosa cells of patients undergoing in vitro fertilisation was investigated. Granulosa cells were collected from 33 patients. Cytosolic free Ca2+ ([Ca2+](i)) concentration, lipid peroxidation, reduced glutathione, glutathione peroxidase and oocyte quality were measured in the granulosa cells. The relationship between two drug protocols was also examined (gonadotrophin-releasing hormone antagonist and agonist protocols) and the same parameters investigated. The [Ca2+](i) concentration (P < 0.001), glutathione (P < 0.05) and oocyte quality (P < 0.001) values were significantly higher in the fertilised group than in the non-fertilised group, although glutathione peroxidase activity was significantly (P < 0.05) higher in the non-fertilised group than in the fertilised group. The [Ca2+](i) concentrations were also higher (P < 0.001) in the good-quality oocyte groups than in the poor-quality oocyte group. There was no correlation between the two drug protocols and investigated parameters. In conclusion, it was observed that high glutathione and cytosolic Ca2+ concentrations in granulosa cells of patients undergoing in vitro fertilisation tended to increase the fertilisation potential of oocytes.