Aim: Acinetobacter spp. have emerged as important nosocomial pathogens in recent decades because of the increase in the incidence of antibiotic-resistant strains. Acinetobacter junii accounts for less than 1% of isolates. Acinetobacter junii is a gram-negative, non-fermentative cocobacillus that might cause bacteremia, especially in immunocompromised patients and newborns. This article described a mini epidemic of Acinetobacter junii that has been experienced in a neonatal intensive care unit (NICU), and taken measures to control it.