The aim of this study is to produce thermoresponsive cotton fabric. For this aim, poly(N-isopropylacrylamide) (PNIPAA) polymer was synthesized and grafted on the cotton fabric by free radical polymerization method. PNIPAA-grafted cotton fabric (PNIPAA-g-CF) was characterized chemically and morphologically and tested to investigate thermoresponsive property. Morphology and chemical structure of PNIPAA-g-CF were investigated by scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR) spectrophotometer analysis, respectively. The results of this study indicated that PNIPAA polymer synthesis and coating on to the cotton fabric surface was realized successfully. Thermoresponsive behaviour of PNIPAA-g-CF was determined by optical microscopy (OM) analysis, and wetting time, water uptake, water vapour permeability and contact angle measurements were performed at different temperature. The results indicated that hydrophilic characteristic of the fabric changed to the hydrophobic depending on increasing temperature. Besides, the pores of the fabric were getting bigger with increasing temperature. All results showed that the PNIPAA-g-CF exhibited thermoresponsive behaviour.