Introduction: Brucellar epididymo-orchitis (BEO) is the most common variant of genitourinary brucellosis and is encountered in 2 to 20% of patients with brucellosis. This report details clinical and laboratory features, diagnostic evaluations, treatment modalities, and final outcomes for 14 patients with brucellar epididymo-orchitis. Material and Methods: In this retrospective study, we describe the medical records of 14 adult patients diagnosed with brucellar epididymo-orchitis between 2009 and 2012. The diagnosis of brucellosis was based on the presence of a positive blood culture or Standard tube agglutination testing with a titer 11160 accompanying positive clinical manifestations. Scrotal pain, enlargement, and swelling with gradual onset were considered as signs of epididymo-orchitis.