Zeolite 4A as a jammer of bacterial communication in Chromobacterium violaceum and Pseudomonas aeruginosa


ULUSOY S., B Akalin R., cevikbas H., Berisha A., ORAL A., TINAZ G.

FUTURE MICROBIOLOGY, vol.17, pp.861-871, 2022 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 17
  • Publication Date: 2022
  • Doi Number: 10.2217/fmb-2021-0174
  • Journal Name: FUTURE MICROBIOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, CAB Abstracts, Chemical Abstracts Core, EMBASE, MEDLINE, Veterinary Science Database
  • Page Numbers: pp.861-871
  • Keywords: biofilms, modeling, Pseudomonas aeruginosa, quorum sensing, QUORUM SENSING INHIBITORS, BIOFILM FORMATION, ESSENTIAL OILS, GENES, DERIVATIVES, ADSORPTION, EXPRESSION, VIRULENCE, ENZYME, STEEL
  • Süleyman Demirel University Affiliated: Yes

Abstract

Aim: To investigate the hypothesis that zeolites interfere with quorum-sensing (QS) systems of Chromobacterium violaceum and Pseudomonas aeruginosa by adsorbing N-acyl homoserine lactone (AHL) signal molecules. Methods: QS inhibition by zeolite 4A was investigated using an AHL-based bioreporter assay. The adsorption of the AHLs was evaluated by performing inductively coupled plasma-optical emission spectroscopy and confirmed by Monte Carlo and molecular dynamic simulations. Results: Zeolite 4A reduced the violacein production in C. violaceum by over 90% and the biofilm formation, elastase and pyocyanin production in P. aeruginosa by 87, 68 and 98%, respectively. Conclusion: Zeolite 4A disrupts the QS systems of C. violaceum and P. aeruginosa by means of adsorbing 3-oxo-C6-AHL and 3-oxo-C12-AHL signaling molecules and can be developed as a novel QS jammer to combat P. aeruginosa-related infections.