© 2021 Taylor & Francis Group, LLC.In this study, the flotation behaviors of gold and gold-associated sulfide minerals (pyrite and sphalerite) in a polymetallic sulfide gold ore were investigated using flotation kinetics parameters. It was found that high gold recovery (>90%) can be achieved by the selective flotation of pyrite and sphalerite. However, the sphalerite flotation results showed that sphalerite could not be separated from pyrite with a high selectivity (selectivity index∼1), because of insufficient depression of pyrite (with high alkaline medium and/or using a depressant). Even if more selective separation was achieved from the pyrite flotation (selectivity index∼5), 40% of sphalerite in the ore was transferred into the pyrite concentrate. In addition, the results of the collectorless tests indicated that more than 80% of the gold can be recovered, and higher selectivity indexes (up to 10) are achievable. Analysis of the particle size distribution of the collectorless flotation concentrate showed that the amount of −40 μm in the concentrate was 90%. In conclusion, a gold concentrate containing low sulfur (10.8%) with a gold recovery of greater than 95% is achievable from a two-stage flow sheet (collectorless flotation as the rougher stage and pyrite flotation as the scavenger stage).