Immunochemical identification of the mammalian peptide hormone obestatin from tea plant (Camellia sinensis)
PAKISTAN JOURNAL OF PHARMACEUTICAL SCIENCES, cilt.36, sa.1, ss.199-204, 2023 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 36 Sayı: 1
- Basım Tarihi: 2023
- Doi Numarası: 10.36721/pjps.2023.36.1.reg.199-204.1
- Dergi Adı: PAKISTAN JOURNAL OF PHARMACEUTICAL SCIENCES
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, CAB Abstracts, EMBASE, MEDLINE, Veterinary Science Database
- Sayfa Sayıları: ss.199-204
- Anahtar Kelimeler: Obestatin-like substances, preproghrelin gene-derived peptides, bioactive peptides, appetite control, tricine-SDS-PAGE, Western blotting, GHRELIN GENE, SECRETION, PROLIFERATION, INSULIN, CELLS, DNA
- Süleyman Demirel Üniversitesi Adresli: Evet
Özet
This study determines obestatin-like substances from the young shoots of the tea plant [Camellia sinensis (L.) O. Kuntze (Theaceae)]. Proteins were extracted from the vegetative tea leaves using the QB (Quick Buffer) buffer as an extraction buffer. Obestatin-like substances in tea extract were investigated using an indirect home-made enzyme-linked immunosorbent assay (ELISA). Human obestatin-like immunoreactive substances from tea extract were isolated and characterized by tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis (tricine-SDS-PAGE) and immunoblotting techniques. Immunochemical results showed that there are strong human obestatin-like immunoreactive substances (0.048 +/- 0.0064 ng/mg protein) in vegetative tea leaves. This finding was completely unexpected since this hormone was considered to be present solely in animals. Furthermore, a single obestatin-like immunoreactive protein band of 13 kDa was identified by tricine-SDS-PAGE and Western blotting of extract of vegetative tea leaf proteins. Present investigation is the first report of presence of obestatin-like immunoreactive substances in plants. It is concluded that obestatin-like bioactive peptides derived from plants can affect gastrointestinal tract structures as endogenous obestatin does and hence play a role in appetite regulation and body weight gain.