Characterization of Exopolysaccharides (EPSs) Obtained from Ligilactobacillus salivarius Strains and Investigation at the Prebiotic Potential as an Alternative to Plant Prebiotics at Poultry


Bikric S., ASLIM B., Dincer I., YÜKSEKDAĞ Z., ULUSOY S. , YAVUZ S.

PROBIOTICS AND ANTIMICROBIAL PROTEINS, 2021 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume:
  • Publication Date: 2021
  • Doi Number: 10.1007/s12602-021-09790-8
  • Title of Journal : PROBIOTICS AND ANTIMICROBIAL PROTEINS
  • Keywords: Ligilactobacillus salivarius, Prebiotic, Anti-biofilm, Anti-quorum sensing, Lactobacilli growth stimulator, Adhesion, INTERNATIONAL SCIENTIFIC ASSOCIATION, LACTOBACILLUS-PLANTARUM, CONSENSUS STATEMENT, BIOFILM FORMATION, PROBIOTICS, GROWTH, INHIBITION, MICROBIOTA, BACTERIA, SCOPE

Abstract

In this study, it was aimed to reveal the potential of using exopolysaccharides (EPS) obtained from Ligilactobacillus salivarius as a prebiotic that regulates chicken intestinal microbiota. Characterization of EPS obtained from L. salivarius BIS312 (EPSBIS312) and BIS722 (EPSBIS722) strains was demonstrated by high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR), and size-exclusion chromatography (SEC) analyses. It was determined that the molecular weight of both EPS is in the range of 10(4)-10(6) Daltons, and there are 4 types of monomers in their structure. Anti-biofilm and anti-quorum sensing effects of EPSBIS312 and EPSBIS722 were determined. EPSBIS312 and EPSBIS722 showed a strong anti-biofilm effect on Enterococcus faecalis ATCC 29212, Staphylococcus aureus EB-1, and Escherichia coli ATCC 11229. The anti-quorum sensing study revealed that the EPSBIS722 had a higher effect than the EPSBIS312. The effect of different concentrations of EPS (2.5%, 5%, 10%) on lactobacilli growth stimulator (LGS) was evaluated. The highest LGS was promoted at 10% concentration while the lowest LGS was promoted at 2.5% concentration by EPSBIS722. In addition, adhesion abilities of EPSBIS312 and EPSBIS722 in HT-29 colorectal adenocarcinoma cell line were tested. EPSs significantly increased the ability to adhere to HT-29 cells. The characterized EPSs may be an alternative to plant prebiotics such as inulin at poultry.