New rRNA primers for the detection of Vibrio anguillarum


Avsever M. L. , Tunaligil S., Didinen B. I. , Metin S.

ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH, cilt.67, 2015 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 67
  • Basım Tarihi: 2015
  • Dergi Adı: ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH

Özet

In this work, 51 V. anguillarum isolates (42/51 of O1 serotype, 9/51 of O2) obtained from cultivated marine fish by Avsever and an (2015) were used. These isolates were confirmed with universal primers specific to amiB genes. They were also detected successfully at a rate of 100% with a new primer couple designed according to 16S ribosomal RNA sequence. The new primers were found to have a high sensitivity and specificity but were liable to give a cross-reaction with Vibrio ordalii.