Purification and partial characterization of xyloglucan-hydrolyzing enzymes from watermelon placental tissue
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, cilt.89, sa.4, ss.645-652, 2009 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 89 Sayı: 4
- Basım Tarihi: 2009
- Doi Numarası: 10.1002/jsfa.3496
- Dergi Adı: JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.645-652
- Süleyman Demirel Üniversitesi Adresli: Evet
Özet
BACKGROUND: Watermelon (Citrulluslonatus(Thunb.) Matsum&Nakai) fruit matrix glycans are comprised largely of xyloglucans (XGs). As in other fruits, these polymers show significant molecular mass downshifts during ripening. In the present study, we describe the purification and characteristics of a number of xyloglucanases (XGases) from the placental tissue of ripe watermelon. XGases were extracted from watermelon fruit placental tissue and purified by sequential ion-exchange, gel-permeation and concanavalin A chromatography.