Purification and partial characterization of xyloglucan-hydrolyzing enzymes from watermelon placental tissue

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Karakurt Y., Huber D. J.

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, vol.89, no.4, pp.645-652, 2009 (SCI-Expanded)

• Publication Type: Article / Article
• Volume: 89 Issue: 4
• Publication Date: 2009
• Doi Number: 10.1002/jsfa.3496
• Journal Name: JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
• Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
• Page Numbers: pp.645-652
• Süleyman Demirel University Affiliated: Yes

Abstract

BACKGROUND: Watermelon (Citrulluslonatus(Thunb.) Matsum&Nakai) fruit matrix glycans are comprised largely of xyloglucans (XGs). As in other fruits, these polymers show significant molecular mass downshifts during ripening. In the present study, we describe the purification and characteristics of a number of xyloglucanases (XGases) from the placental tissue of ripe watermelon. XGases were extracted from watermelon fruit placental tissue and purified by sequential ion-exchange, gel-permeation and concanavalin A chromatography.